Journal: bioRxiv

Article Title: Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

doi: 10.1101/2022.06.17.496630

Figure Lengend Snippet: Key resource table

Article Snippet: CD86 Antibody, anti-human, PE, REAfinityTM (Clone- REA968) , Miltenyi Biotec , Order Number: 130-116-160.

Techniques: Recombinant, Sterility, Injection, Modification, Staining, Saline, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Software

Journal: Redox Biology

Article Title: Regulation of type I interferon responses by mitochondria-derived reactive oxygen species in plasmacytoid dendritic cells

doi: 10.1016/j.redox.2017.07.016

Figure Lengend Snippet: Phenotypical analysis of AMA-exposed GEN2.2 cell. Cells were treated with AMA (0.5 μg/ml) and CpG-A (1 μM) separately and in combination or were left untreated. Following 6 h of stimulation the expression levels of CD40 (A), CD80 (B), CD86 (C) and HLA-DQ (D) cell surface proteins were analyzed by flow cytometry. Relative fluorescence intensity values were calculated using the respective isotype-matched control antibodies. The bars represent fold changes compared to the untreated control and data are expressed as the mean ± SD of four independent experiments. *p < 0.05 vs. control. AMA: Antimycin-A.

Article Snippet: After 24 h of treatments the phenotypical analysis of the cells was performed by flow cytometry using FITC-labeled monoclonal antibodies against CD40 and CD80 (Cat. no. 334306 and 305206 both from BioLegend, San Diego, CA, USA), PE-labeled CD86 (Cat. no. FAB141P from R&D System, Minneapolis, MN, USA), HLA-DQ (Cat. no. 318106 from BioLegend) and isotype-matched control antibodies (all from BioLegend).

Techniques: Expressing, Flow Cytometry, Fluorescence

Journal: Pharmaceutics

Article Title: Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination

doi: 10.3390/pharmaceutics14102000

Figure Lengend Snippet: Flow cytometric analysis of CD11c, CD80, and CD86 expression in BMDCs after treatment of blank medium, free DG, and DG-P for 24 h ( n = 4).

Article Snippet: Fluorochrome-labelled anti-mouse monoclonal antibodies including APC-CD11c, FITC-CD80, and PE-CD86, were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing

Journal: Pharmaceutics

Article Title: Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination

doi: 10.3390/pharmaceutics14102000

Figure Lengend Snippet: Quantification of CD80 and CD86 expression on BMDCs after activation ( n = 4). Note: *, ** and *** represent the differences at p < 0.05, p < 0.01, and p < 0.001, respectively.

Article Snippet: Fluorochrome-labelled anti-mouse monoclonal antibodies including APC-CD11c, FITC-CD80, and PE-CD86, were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Activation Assay

Journal: Cell Reports Medicine

Article Title: Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibition

doi: 10.1016/j.xcrm.2024.101830

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-CD86 , Miltenyi Biotec , Cat#130-123-724,;RRID: AB_2889634.

Techniques: Staining, Virus, Recombinant, Bicinchoninic Acid Protein Assay, Cell Viability Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Blocking Assay, Phospho-proteomics, Purification, Mass Spectrometry, Software, Modification

Journal: Biomedicines

Article Title: Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes

doi: 10.3390/biomedicines8120627

Figure Lengend Snippet: Antibodies used in the flow cytometry assay.

Article Snippet: CD86-PE-Vio770, mouse , Rat IgG2b-PE-Vio770 , Miltenyi Biotec.

Techniques: Flow Cytometry, Control

Journal: Journal of Experimental Orthopaedics

Article Title: Neutralization of HMGB1 improves fracture healing and γδ T lymphocyte counts at the fracture site in a polytrauma rat model

doi: 10.1186/s40634-022-00453-3

Figure Lengend Snippet: Antibodies and their titrated dilutions used for flow cytometry

Article Snippet: Anti-rat CD86 antibody , Miltenyi Biotech , 1:10.

Techniques: Blocking Assay